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cd14 antibody, anti-human  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 antibody, anti-human
    Cd14 Antibody, Anti Human, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 674 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+anti+human/CD14+Antibody%2C+anti-human/custom%40130-113-708%4042727576
    Average 96 stars, based on 674 article reviews
    cd14 antibody, anti-human - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Labeling:

    Article Title: Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibition.
    Article Snippet: Cells were cultured on 6 well plates or 10cm dishes from Corning and incubated with 5% CO2 at 37,0 C. The cells were cultured in RPMI 1640 medium containing 10% FBS and 1% penicillin/streptomycin. .. Monocytes of buffy coats were separated by CD14 anti-human magnetically labeled MicroBeads from Miltenyi Biotec. ..

    Article Title: Macrophages are activated toward phagocytic lymphoma cell clearance by pentose phosphate pathway inhibition
    Article Snippet: .. Monocytes of buffy coats were separated by CD14 anti-human magnetically labeled MicroBeads from Miltenyi Biotec. ..

    Article Title: Short-term Flt3L treatment effectively mobilizes functional macaque dendritic cells.
    Article Snippet: .. This was achieved using the Miltenyi AutoMACS System (Miltenyi Biotec, Auburn, CA), where cells were first labeled with fluorescein isothiocyanate (FITC)conjugated antibodies (Ab) against CD3 (clone SP34); CD8 (clone SK1) and CD20 (clone L27), followed by incubation with anti-FITC, -CD11b (antimouse/human), -CD14 (anti-human), and -CD19 (anti-human) beads (Miltenyi Biotec); and collection of the flow-through Lin fraction (N. Teleshova et al., submitted). ..

    Article Title: Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro
    Article Snippet: .. Add 200 μL CD14 anti-human magnetically labeled MicroBeads. k. Add 800 μL of MACS buffer (4°C). l. Perform magnetic separation on a flow cytometer (e.g., MacsQuant X flow cytometer with Miltenyi Biotec CD14 human microbead isolation protocol). m. Re-suspend CD14 + cells in non-supplemented RPMI 1640 media immediately. ..

    Incubation:

    Article Title: Short-term Flt3L treatment effectively mobilizes functional macaque dendritic cells.
    Article Snippet: .. This was achieved using the Miltenyi AutoMACS System (Miltenyi Biotec, Auburn, CA), where cells were first labeled with fluorescein isothiocyanate (FITC)conjugated antibodies (Ab) against CD3 (clone SP34); CD8 (clone SK1) and CD20 (clone L27), followed by incubation with anti-FITC, -CD11b (antimouse/human), -CD14 (anti-human), and -CD19 (anti-human) beads (Miltenyi Biotec); and collection of the flow-through Lin fraction (N. Teleshova et al., submitted). ..

    Magnetic Cell Separation:

    Article Title: Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro
    Article Snippet: .. Add 200 μL CD14 anti-human magnetically labeled MicroBeads. k. Add 800 μL of MACS buffer (4°C). l. Perform magnetic separation on a flow cytometer (e.g., MacsQuant X flow cytometer with Miltenyi Biotec CD14 human microbead isolation protocol). m. Re-suspend CD14 + cells in non-supplemented RPMI 1640 media immediately. ..

    Flow Cytometry:

    Article Title: Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro
    Article Snippet: .. Add 200 μL CD14 anti-human magnetically labeled MicroBeads. k. Add 800 μL of MACS buffer (4°C). l. Perform magnetic separation on a flow cytometer (e.g., MacsQuant X flow cytometer with Miltenyi Biotec CD14 human microbead isolation protocol). m. Re-suspend CD14 + cells in non-supplemented RPMI 1640 media immediately. ..

    Isolation:

    Article Title: Protocol for assessing antibody-mediated cellular phagocytosis of lymphoma cells by human and murine macrophages in vitro
    Article Snippet: .. Add 200 μL CD14 anti-human magnetically labeled MicroBeads. k. Add 800 μL of MACS buffer (4°C). l. Perform magnetic separation on a flow cytometer (e.g., MacsQuant X flow cytometer with Miltenyi Biotec CD14 human microbead isolation protocol). m. Re-suspend CD14 + cells in non-supplemented RPMI 1640 media immediately. ..



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    Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) <t>CD14+</t> monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.
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    Miltenyi Biotec resource source identifier antibodies anti cd14 percp vio700 miltenyi biotech
    Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) <t>CD14+</t> monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.
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    Image Search Results


    Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.

    Journal: Physiological Reports

    Article Title: Sex differences in circulating platelet‐derived CD41 + extracellular vesicles in healthy adults

    doi: 10.14814/phy2.70932

    Figure Lengend Snippet: Nano flow cytometry measures of MV phenotype in males and females. (a) CD3+ lymphocyte‐derived MVs. (b) CD14+ monocyte‐derived MVs. (c) CD16+ neutrophil‐derived MVs. (d) CD45+ leukocyte‐derived MVs. (e) CD31+ endothelial cell‐derived MVs. (f) CD62E+ endothelial activation‐derived MVs. (g) CD41+ platelet‐derived MVs. (h) MVs expressing Annexin A5. Individual data points are presented as MV counts/μL. Clear circles represent females and dark filled circles represent males. Statistical comparisons between sexes were performed using linear models adjusted for age and BMI. Data are presented as untransformed values for visualization following outlier removal using a 3*SD criterion. Statistical analyses were performed on log‐transformed data where appropriate.

    Article Snippet: Panel 2 received 2 μL each of CD3 PE (130‐114‐519, Miltenyi Biotec), CD14 PerCP‐Vio700 (130‐110‐523), CD16 PE‐Vio615 (130‐119‐995), CD31 FITC (130‐110‐668), CD41 APC (130‐123‐301), and 10 μL of CD62E PE (130‐104‐643).

    Techniques: Flow Cytometry, Derivative Assay, Activation Assay, Expressing, Transformation Assay